高良姜素对人胃癌SGC-7901细胞增殖、周期循环和凋亡的体外影响

许云霞,赵新淮

中国药学杂志 ›› 2013, Vol. 48 ›› Issue (15) : 1274-1278.

中国药学杂志 ›› 2013, Vol. 48 ›› Issue (15) : 1274-1278. DOI: 10.11669/cpj.2013.15.010
论著

高良姜素对人胃癌SGC-7901细胞增殖、周期循环和凋亡的体外影响

  • 许云霞,赵新淮
作者信息 +

In Vitro Effects of Galangin on Cell Proliferation,Cycle Progression and Apoptosis of a Human Gastric Cancer SGC-7901 Cells

  • XU Yun-xia,ZHAO Xin-huai
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摘要

目的探讨高良姜素对人胃癌SGC-7901细胞增殖抑制和凋亡诱导作用及可能机制。方法采用噻唑蓝(MTT)法检测细胞增殖活性,流式细胞仪、形态学方法和线粒体膜电位分析高良姜素对SGC-7901细胞周期循环和凋亡的影响。结果在作用浓度为40~200μmol·L-1时,高良姜素对SGC-7901细胞有显著的增殖抑制作用,并呈现剂量-效应和时间-效应依赖关系;作用24、48和72h时,半数抑制浓度(IC50)分别是160、100和70μmol·L-1;高良姜素作用24h后,G2/M期细胞比例从4.40%增加到18.31%。高良姜素作用后细胞呈现典型的凋亡形态学特征,线粒体膜电位明显降低,160μmol·L-1高良姜素作用24、48h时,凋亡细胞比例从2.6%和4.3%增加至27.4%和65.6%。结论高良姜素可能通过干扰细胞周期循环和介导线粒体功能障碍,诱导细胞凋亡,从而抑制胃癌SGC-7901细胞的生长。

Abstract

OBJECTIVE To investigate the effect of galangin on the anti-proliferation and apoptosis of gastric cancer SGC-7901 cells,and possible mechanisms. METHODS Proliferative activity of SGC-7901 cells was measured by MTT assay while that cell cycle progression and apoptosis of galangin-treated SGC-7901 cells were analyzed by flow cytometry,morphologic observation and mitochondrial membrane potential(MMP) analysis. RESULTSS The evaluated results showed that when galangin was added into cell culture in 40-200 μmol·L-1,proliferative inhibition occurred and was observed as a dose- and time-dependent manner. The calculated IC50 values for galangin were 160,100 and 70 μmol·L-1 when the treatment time was 24,48 and 72 h,respectively. Flow cytometry analysis revealed that the proportion of the cells in the G2/M phase was significantly enhanced from 4.40(control group) to 18.31(treatment group) by a treatment time of 24 h.CONCLUSION The cells in treatment group showed typical apoptotic morphology and a decrease in MMP. At the same time,the percentage of the apoptotic cells significantly increased from 2.6% or 4.3%(control group) to 27.4% or 65.6%(treatment group),when 160 μmol·L-1 galangin treated SGC-7901 cells for 24 or 48 h. These mentioned results indicate that galangin can inhibit the proliferation and induce apoptosis of the SGC-7901 cells by perturbation in cell cycle progression and mitochondrial dysfunction.

关键词

高良姜素 / 人胃癌细胞 / 增殖抑制 / 周期停滞 / 凋亡

Key words

galangin / human gastric cancer cell line / proliferative inhibition / cell cycle arrest / apoptosis

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导出引用
许云霞,赵新淮. 高良姜素对人胃癌SGC-7901细胞增殖、周期循环和凋亡的体外影响[J]. 中国药学杂志, 2013, 48(15): 1274-1278 https://doi.org/10.11669/cpj.2013.15.010
XU Yun-xia,ZHAO Xin-huai. In Vitro Effects of Galangin on Cell Proliferation,Cycle Progression and Apoptosis of a Human Gastric Cancer SGC-7901 Cells[J]. Chinese Pharmaceutical Journal, 2013, 48(15): 1274-1278 https://doi.org/10.11669/cpj.2013.15.010
中图分类号: R965   

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基金

黑龙江省教育厅科学技术研究重点项目计划(11551z018)

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